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Romanian Journal of Biology - Plant Biology
ROMANIAN ACADEMY |
INFLUENCE OF CULTURE MEDIUM COMPOSITION ON THE PROTEOLYTIC ACTIVITY OF THE HALOTOLERANT BACTERIAL STRAIN SALINIVIBRIO COSTICOLA SUBSP. ALKALIPHILUS IBB 37
ALINA BADEA (CIOACĂ), SIMONA NEAGU, MĂDĂLIN ENACHE, VERONICA LAZĂR
Abstract:
Microbial proteases are important biocatalysts with broad industrial and environmental applications, and halophilic strains offer robust enzymes capable of functioning under extreme conditions. This study investigated the influence of culture medium pH and glucose concentration on the production and activity of an extracellular protease synthesized by the halotolerant bacterium Salinivibrio costicola subsp. alkaliphilus IBB37, isolated from a mud sample. The strain was cultivated in a medium containing 10% NaCl at varying pH values (5, 6, 7, and 8) and supplemented with either 5% or 10% glucose and 1% casein as the substrate. Results showed that alkaline conditions greatly enhanced protease yield: the highest proteolytic activity was obtained at pH 8 with 5% glucose, whereas lower pH or higher glucose (10%) led to reduced enzyme activity. Under the optimal pH 8 and 5% glucose medium, the protease's specific activity increased approximately nine-fold after purification compared to the crude extract. Efficient production and purification of this halotolerant protease underscore its potential for biotechnological applications, such as in high-salinity bioprocesses, waste management, or agricultural soil amendments, where robust salt-tolerant enzymes are required.
Citation:
ALINA BADEA (CIOACĂ), SIMONA NEAGU, MĂDĂLIN ENACHE, VERONICA LAZĂR, 2025. INFLUENCE OF CULTURE MEDIUM COMPOSITION ON THE PROTEOLYTIC ACTIVITY OF THE HALOTOLERANT BACTERIAL STRAIN SALINIVIBRIO COSTICOLA SUBSP. ALKALIPHILUS IBB 37, Romanian Journal of Biology - Plant Biology, 70(1-2): 67–74. DOI: DOI: 10.59277/RJB-PB.2025.1-2.05.
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